Estandarización del método de selección de gametos en la F1 de cruzas dobles para resistencia múltiple a enfermedades en fréjol común (Phaseolus vulgaris L.). Santa Catalina, INIAP, 2006
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Sangolquí, EC: Escuela Politécnica del Ejército, Facultad de Ciencias Agropecuarias, 2007. 78 p.
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E. E. Santa Catalina
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3 p.
Abstract
El presente estudio tuvo como primer objetivo la evaluación de poblaciones Fi de fréjol
común mediante inoculaciones secuenciadas de C. lindemuthianum, U. appendiculatus
y P. griseola para identificar individuos con resistencia múltiple. Adicionalmente, un
segundo objetivo fue la estandarización de una metodología de inoculación secuenciada.
Finalmente, el marcador molecular SAE19 fue evaluado. Este marcador está ligado al
gen de resistencia para roya, Ur-11. Un total de 83 individuos fueron seleccionados por
presentar resistencia múltiple a las enfermedades bajo estudio. La secuencia de
inoculación, que no causó interacción entre las especies de patógenos, fue C.
lindemuthianum-U. appendiculatus-P. griseola, siendo esta seleccionada para formar
parte de la rutina de evaluación del Programa Nacional de Leguminosas y Granos
Andinos del INIAP. El marcador SCAR no fue aplicable a las poblaciones bajo estudio,
debido a que los progenitores no presentaron polimorfismos asociados a la resistencia y
susceptibilidad.
The present research had as first objective the evaluation of a F| common bean population, through sequenced inoculations of C. lindemuthianum, U. appendiculatus and P. griseola to identify genotypes with múltiple resistances. Additionally, a second objective was the standardization of sequenced inoculation methodology. Finally, the molecular marker SAE19 was evaluated. This marker is linked to the resistance gene Ur-11 for rust. A total of 83 genotypes were selected for having múltiple resistances to the diseases under study. The inoculation sequence that did not make interaction among pathogens was C. lindemuthianum-U. appendiculatus-P. griseola, this one was selected to be part of Legume and Andean Grains National Program- INIAP evaluation routine. The SCAR marker was not able to applied on the populations under study, due to the parental genotypes did not harbor polymorphism linked to resistance and susceptibility.
The present research had as first objective the evaluation of a F| common bean population, through sequenced inoculations of C. lindemuthianum, U. appendiculatus and P. griseola to identify genotypes with múltiple resistances. Additionally, a second objective was the standardization of sequenced inoculation methodology. Finally, the molecular marker SAE19 was evaluated. This marker is linked to the resistance gene Ur-11 for rust. A total of 83 genotypes were selected for having múltiple resistances to the diseases under study. The inoculation sequence that did not make interaction among pathogens was C. lindemuthianum-U. appendiculatus-P. griseola, this one was selected to be part of Legume and Andean Grains National Program- INIAP evaluation routine. The SCAR marker was not able to applied on the populations under study, due to the parental genotypes did not harbor polymorphism linked to resistance and susceptibility.