Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.iniap.gob.ec/handle/41000/4508
Tipo de Documento: Revista
Artículo
Autor : Fu, Zhen
Epstein, Brendan
Kelley, Joanna L.
Zheng, Qi
Bergland, Alan O.
Castillo C., Carmen
Jensen, Andrew S.
Dahan, Jennifer
Karasev, Alexander V.
Snyder, William E.
Título : Using NextRAD sequencing to infer movement of herbivores among host plants
Título de la serie: Plos One 12(5):1-19
Palabras clave : SOLANUM DULCAMARA;PLAGAS;BACTERICERA COCKERELLI;HOSPEDEROS ALTERNOS;NGS;SECUENCIACIÓN
Fecha de publicación : 15-may-2017
Código: *EC-INIAP-BEESC-MGC. Quito (INIAP/CD192CC)
Páginas: p. 1-19
Estación: E. E. Santa Catalina
Resumen : Herbivores often move among spatially interspersed host plants, tracking high-quality resources through space and time. This dispersal is of particular interest for vectors of plant pathogens. Existing molecular tools to track such movement have yielded important insights, but often provide insufficient genetic resolution to infer spread at finer spatiotemporal scales. Here, we explore the use of Nextera-tagmented reductively-amplified DNA (NextRAD) sequencing to infer movement of a highly-mobile winged insect, the potato psyllid (Bactericera cockerelli), among host plants. The psyllid vectors the pathogen that causes zebra chip disease in potato (Solanum tuberosum), but understanding and managing the spread of this pathogen is limited by uncertainty about the insect’s host plant(s) outside of the growing season. We identified 1,978 polymorphic loci among psyllids separated spatiotemporally on potato or in patches of bittersweet nightshade (S. dulcumara), a weedy plant proposed to be the source of potato-colonizing psyllids. A subset of the psyllids on potato exhibited genetic similarity to insects on nightshade, consistent with regular movement between these two host plants. However, a second subset of potato-collected psyllids was genetically distinct from those collected on bittersweet nightshade; this suggests that a currently unrecognized source, i.e., other nightshade patches or a third host-plant species, could be contributing to psyllid populations in potato. Oftentimes, dispersal of vectors of pathogens must be tracked at a fine scale in order to understand, predict, and manage disease spread. We demonstrate that emerging sequencing technologies that detect genome-wide SNPs of a vector can be used to infer such localized movement.
Citación : Fu, Z., Epstein, B., Kelley, J. L., Zheng, Q., Bergland, A. O., Castillo C., C., ... Snyder, W. E. (may, 2017). Using NextRAD sequencing to infer movement of herbivores among host plants. Plos One, 12(5), 1-19. https://doi.org/10.1371/journal.pone.0177742.
URI : http://repositorio.iniap.gob.ec/handle/41000/4508
Aparece en las colecciones: Artículos de Revistas EESC

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
iniapscCD192CC.pdf657,33 kBAdobe PDFVista previa
Visualizar/Abrir


Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons